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Just to add to the previous points - I've found that, nearly all the time, leaks have actually been around the exit/entry ports and that you need to be careful that no kinks in the bagging film occur around these areas when pulling down the bag
I create a donut ring of tape on top of each silicone connector and carefully tape the bag down onto it when I reach that point when sticking down the perimeter. Again, be careful that there's enough slack around the connector so not to cause excessive pulling of the bag here when it's vacuumed and that everything doesn't wrinkle up when you tape around the tubing to finish (be careful when putting the tube in also) The donut ring doesn't seal the connector off, but it seems to help to prevent too much wrinkling of the bag here and allow you the chance to smooth out the bag
As leaks in this area are typically very small, your pump will pull down the bag fully and might give the impression of total vacuum but, annoyingly sometimes, a tiny leak only becomes apparent once the infusion is done and you start to see a very slow creep of air around this point.
If I don't get reliable vacuum after going around the whole bag a couple of times I now tend to take off the tape around the inlet/outlet tubing and re-do, making sure the bagging film is as smooth as possible. This appears to fix things most of the time for me
I've also started putting a strip of peel ply over the infusion spiral too, as I've experienced one or two leaks pop up here that only seem to become apparent after starting the infusion as well.
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